Study on medication rule of TCM fumigation and washing in promoting the postoperative healing of hemorrhoids / 国际中医中药杂志

Objective:To analyze the medication law of Traditional Chinese Medicine (TCM) fumigation and washing to promote postoperative healing of hemorrhoids by data mining technology.Methods:The clinical literature about TCM fumigation and washing to promote postoperative healing of hemorrhoids was retrieved from the databases of CNKI, Wanfang, VIP, PubMed from the establishment of the databases to March 10, 2022. The frequency efficacy attributes, core medicinal pairs and core prescriptions of TCM were analyzed by using the Ancient and Modern Medical Records Cloud Platform (V2.3.5).Results:A total of 299 articles were included, involving 200 kinds of Chinese materia medica. The drugs used at high frequency ≥40 were Sophorae Flavescentis Radix, Phellodendri Chinensis Cortex, Natrii Sulfas, Galla Chinensis and Rhei Radix et Rhizoma and so on. The main efficacy was to clear heat and reduce dampness; cold, warm and slightly cold were the main medicinal properties, and the tastes were mainly bitter, pungent, sweet and sour, and most of the drugs return to the liver meridian, stomach meridian, heart meridian, large intestine meridian and so on. A total of 22 rules were obtained by correlation analysis. Five groups of drugs were obtained by clustering analysis. The core prescription drugs obtained by complex network analysis included Sophorae Flavescentis Radix, Phellodendri Chinensis Cortex, Natrii Sulfas, Galla Chinensis, Rhei Radix et Rhizoma, Taraxaci Herba, Borneolum Syntheticum, Sanguisorbae Radix, Atractylodis Rhizoma, Carthami Flos, Scutellariae Radix, Olibanum, Myrrha, and Lonicerae Japonicae Flos. Conclusion:TCM fumigation and washing can promote the postoperative healing of hemorrhoids mainly by clearing heat and reducing dampness and detoxification, as well as promoting blood circulation and removing blood stasis, reducing swelling and relieving pain, restraining sore and generating muscle.

WD repeat domain 5 promotes the development of late-onset preeclampsia by activating nuclear factor kappa B

Purpose: Over-activation of nuclear factor kappa B (NF-κB) was proven to be involved in the pathogenesis of preeclampsia. However, its regulation mechanism is not clear yet. This paper explored the role of WD repeat domain 5 (WDR5) in the development of late-onset preeclampsia and its relationship with NF-κB. Methods: WDR5 expression was detected in normal placentas and placentas from late-onset preeclampsia patients. CCK-8 and colony formation assays were conducted to appraise the proliferative ability of trophoblast. Migration and invasion were observed by wound healing and transwell assays. The interaction between WDR5 and NF-κB inhibitor I-kappa-B-alpha (IkBa) was verified by Co-immunoprecipitation analysis. Immunofluorescence was used to analyze the activation of NF-κB. Finally, we tested the role of WDR5 using the mice late-onset preeclampsia model. Results: WDR5 was highly expressed in the placentas of late-onset preeclampsia patients. WDR5 overexpression suppressed cell proliferation, migration, and invasion in trophoblast. WDR5 could interact with IkBa to activate NF-κB. Knockdown of NF-κB counteracted the anti-proliferative and anti-metastatic effects of WDR5 overexpression in trophoblast. In-vivo studies suggested that targeting WDR5 combated late-onset preeclampsia development. Conclusions: Our finding provides new insights into the role of WDR5 in late-onset preeclampsia development.

Analysis of causes and prevention of ankle injuries in college basketball / Análise das causas e prevenção contra lesões no tornozelo no basquetebol universitário / Análisis de las causas y la prevención de las lesiones de tobillo en el baloncesto universitario

ABSTRACT Introduction: Basketball is a combative sport, very popular among college students. However, it requires many periods of running, interspersed with emergency breaks, tackling, and other actions prone to ankle injuries. Objective: Investigate the causes of ankle joint injuries in college basketball, raising countermeasures to prevent these sports injuries. Methods: 413 college students who regularly participate in basketball games at 10 colleges and universities were selected as research objects. The causes of ankle joint injuries in the process of this sport were investigated through questionnaires, and the main causes of injuries were analyzed using statistical methods. Results: The causes of ankle joint injuries in college basketball include subjective and objective factors. Subjective factors include inadequate psychological preparation, warm-up exercise, cushioning, and excessive exercise, among others listed; objective factors mainly contemplate local injury and accidental injury. Conclusion: The participation of college students in basketball is beneficial to improve their physical health, requiring effective measures to prevent possible ankle injuries, both related to subjective and objective aspects. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução: o basquetebol é uma modalidade esportiva combativa, muito popular entre os universitários. No entanto, necessita de muitos períodos de corrida, intercalados por pausas emergenciais, enfrentamentos e outras ações propensas a lesões no tornozelo. Objetivo: Investigar as causas de lesões nas articulações do tornozelo no basquetebol universitário, levantando contramedidas para prevenir essas lesões desportivas. Métodos: O total de 413 estudantes universitários que participam regularmente em jogos de basquetebol em 10 faculdades e universidades foram selecionados como objetos de pesquisa. As causas das lesões nas articulações do tornozelo no processo desse esporte foram investigadas através de questionários, sendo analisadas as principais causas das lesões através de métodos estatísticos. Resultados: as causas das lesões nas articulações do tornozelo dentro do basquetebol universitário incluem principalmente fatores subjetivos e objetivos. Os fatores subjetivos incluem a preparação psicológica inadequada, o exercício de aquecimento inadequado, o amortecimento inadequado, o exercício excessivo, entre outros listados; os fatores objetivos contemplam principalmente a lesão local e a lesão acidental. Conclusão: A participação dos estudantes universitários no basquetebol é benéfica para melhorar a sua saúde física, requerendo medidas eficazes para prevenir possíveis lesões no tornozelo, tanto as relacionadas aos aspectos subjetivos como objetivos. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción: El baloncesto es una modalidad deportiva combativa, muy popular entre los estudiantes universitarios. Sin embargo, requiere muchos periodos de carrera, intercalados con pausas de emergencia, enfrentamientos y otras acciones propensas a las lesiones de tobillo. Objetivo: Investigar las causas de las lesiones de la articulación del tobillo en el baloncesto universitario, planteando contramedidas para prevenir estas lesiones deportivas. Métodos: Se seleccionaron como objeto de investigación un total de 413 estudiantes universitarios que participaban regularmente en partidos de baloncesto en 10 colegios y universidades. Las causas de las lesiones de la articulación del tobillo en el proceso de este deporte se investigaron mediante cuestionarios, y las principales causas de las lesiones se analizaron mediante métodos estadísticos. Resultados: Las causas de las lesiones de la articulación del tobillo en el baloncesto universitario incluyen principalmente factores subjetivos y objetivos. Los factores subjetivos incluyen una preparación psicológica inadecuada, un ejercicio de calentamiento inadecuado, una amortiguación inadecuada, un ejercicio excesivo, entre otros enumerados; los factores objetivos contemplan principalmente la lesión local y la lesión accidental. Conclusión: La participación de los estudiantes universitarios en el baloncesto es beneficiosa para mejorar su salud física, requiriendo medidas eficaces para prevenir posibles lesiones de tobillo, tanto las relacionadas con aspectos subjetivos como objetivos. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Changes in vo2max caused by aerobic exercise in swimmers / Alterações de vo2max ocasionadas pelo exercício aeróbico em nadadores / Cambios en el vo2max causados por el ejercicio aeróbico en nadadores

ABSTRACT Introduction The maximal oxygen consumption rate (VO2max) can directly reflect the body's aerobic metabolism and oxygen delivery system. This is a measure of aerobic capacity used to monitor swimmers' levels and their strength and speed performance. Objective Verify the changes in VO2max when incorporating aerobic exercise training into swimmers' regular training. Methods 12 volunteer swimmers were divided into two categories: strength training group and regular training group. Both groups performed a fitness test on the 200-meter track before starting the training. VO2 maximal was measured and worked out statistically to verify the results of this competition. The effects of aerobic exercise on the strength and competitive status of the athletes were investigated according to an updated literature review. Results There was a significant change in the maximal oxygen uptake of the athletes after the experimental protocol. The athletes in the strength training group increased their VO2 max compared to the regular training group. The results showed a significant difference in the VO2max index before and after strength training (P<0.05). This suggests that strength training has a significant effect on maximal oxygen consumption. Conclusion Strength training interventions significantly affect athletes' maximal oxygen consumption intensity and exercise capacity. The aerobic exercise intervention showed evidence of improving the competitive level of athletes. Level of evidence II; Therapeutic studies -investigation of treatment outcomes.

RESUMO Introdução A taxa máxima de consumo de oxigênio (VO2max) pode refletir diretamente o metabolismo aeróbico corporal e o sistema de fornecimento de oxigênio. Esta é uma medida da capacidade aeróbica empregada para monitorar o nível do nadador, e seu desempenho em força e velocidade. Objetivo Verificar as alterações de VO2max ao incorporar o treinamento com exercícios aeróbicos no treino padrão dos nadadores. Métodos 12 nadadores voluntários foram divididos em duas categorias: grupo de treinamento de força e grupo de treinamento regular. Ambos os grupos realizaram um teste de aptidão física na pista de 200 metros antes de iniciar o treinamento. O VO2 máximo foi mensurado e trabalhado estatisticamente para verificar os resultados desta competição. Os efeitos do exercício aeróbico sobre a força e o status competitivo dos atletas foi investigado segundo uma revisão bibliográfica atualizada. Resultados Houve uma mudança significativa na ingestão máxima de oxigênio dos atletas após o protocolo experimental. Os atletas do grupo de treinamento de força aumentaram seu VO2 máximo quando comparados ao grupo de treinamento regular. Os resultados mostraram uma diferença significativa no índice VO2max antes e depois do treinamento de força (P<0,05). Isto sugere que o treinamento de força tem um efeito significativo no consumo máximo de oxigênio. Conclusão As intervenções de treinamento de força afetam significativamente a intensidade máxima de consumo de oxigênio e a capacidade de exercício dos atletas. A intervenção do exercício aeróbico demonstrou evidencias em melhorar o nível competitivo dos atletas. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción La tasa máxima de consumo de oxígeno (VO2máx) puede reflejar directamente el metabolismo aeróbico del cuerpo y el sistema de suministro de oxígeno. Se trata de una medida de la capacidad aeróbica que sirve para controlar el nivel del nadador y su rendimiento en fuerza y velocidad. Objetivo Verificar los cambios en el VO2máx al incorporar el entrenamiento de ejercicio aeróbico en el entrenamiento habitual de los nadadores. Métodos 12 nadadores voluntarios fueron divididos en dos categorías: grupo de entrenamiento de fuerza y grupo de entrenamiento regular. Ambos grupos realizaron una prueba de aptitud física en 200 metros de pista antes de comenzar el entrenamiento. Se midió el VO2 máximo y se trabajó estadísticamente para verificar los resultados de esta competición. Se investigaron los efectos del ejercicio aeróbico en la fuerza y el estado competitivo de los atletas según una revisión bibliográfica actualizada. Resultados Hubo un cambio significativo en el consumo máximo de oxígeno de los atletas después del protocolo experimental. Los atletas del grupo de entrenamiento de fuerza aumentaron su VO2 máximo en comparación con el grupo de entrenamiento regular. Los resultados mostraron una diferencia significativa en el índice VO2max antes y después del entrenamiento de fuerza (P<0,05). Esto sugiere que el entrenamiento de fuerza tiene un efecto significativo en el consumo máximo de oxígeno. Conclusión Las intervenciones de entrenamiento de fuerza afectan significativamente a la intensidad del consumo máximo de oxígeno y a la capacidad de ejercicio de los atletas. La intervención de ejercicio aeróbico mostró evidencia de mejorar el nivel competitivo de los atletas. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Astragaloside IV alleviates lung inflammation in Klebsiella pneumonia rats by suppressing TGF-β1/Smad pathway

Astragaloside IV is a biologically active substance derived from the traditional Chinese medicine Astragalus mambranaceus Bunge, and has antioxidant, anti-inflammatory, and anti-apoptotic properties. In this study, we aimed to investigate the effects of astragaloside IV on Klebsiella pneumonia rats and the underlying mechanisms. Klebsiella pneumoniae (K. pneumoniae) rats were treated with different dosages of astragaloside IV (5, 10, and 20 mg/kg) by intragastric administration. The levels of pro-inflammatory cytokines interleukin (IL)-6, IL-1β, and tumor necrosis factor (TNF)-α in bronchoalveolar lavage fluid (BALF) were determined. Pathological changes of lung tissue were inspected by HE staining. The expression of transforming growth factor (TGF)-β1 in lung tissue was determined with immunohistochemistry, and the expression levels of TGF-β1, p-Smad2/Smad2, p-Smad3/Smad3, IκBα/p-IκBα, and p65/p-p65 in lung tissue were determined by western blot. The mechanism was further investigated with TGF-β1 inhibitor SB-431542. Astragaloside IV reduced the elevated levels of pro-inflammatory cytokines caused by K. pneumoniae and improved lung tissue damage in a dose-dependent manner. Astragaloside IV also decreased the expression of TGF-β1/Smad signaling pathway-related proteins and decreased the protein levels of inflammation-related p-IκBα and p65 in lung tissues induced by K. pneumoniae. Additionally, it was found that the effects of 20 mg/kg astragaloside IV were similar to SB-431542, which could improve pulmonary fibrosis induced by K. pneumoniae, decrease the levels of TGF-β1/Smad signaling pathway-related proteins in lung, and reduce inflammation at the same time. Astragaloside IV could alleviate the inflammation of rat pneumonia induced by K. pneumoniae through suppressing the TGF-β1/Smad pathway.

Bibliometric analysis of publications pertaining to artemisinin resistance in Plasmodium falciparum based on Web of Science from 2011 to 2022 / 中国血吸虫病防治杂志

Objective To analyze the global literature output and citation of publications pertaining to artemisinin (ART) resistance in Plasmodium falciparum from 2011 to 2021, so as to provide insights into researches on resistance of P. falciparum to ART. Methods The publications pertaining to ART resistance in P. falciparum were retrieved from the Science Citation Index Expanded (SCIE) database in Web of Science during the period from January 2011 through May 2022. The subject, journal, country, affiliation and author distributions and citations of publications were descriptively analyzed. Results A total of 1 640 publications pertaining to ART resistance in P. falciparum were retrieved in the SCIE database during the period from January 2011 through May 2022, and the number of publications appeared an overall tendency towards a rise during the study period. These articles were published in 343 journals, and the three most productive journals included Malaria Journal (341 publications, 20.79%), Antimicrobial Agents and Chemotherapy (141 publications, 8.60%), American Journal of Aropical Medicine and Hygiene (68 publications, 4.15%), with infectious diseases (565 publications, 34.45%), parasitology (531 publications, 32.38%), and tropical medicine (517 publications, 31.54%) as the predominant subject. The three most productive countries included the United States of America (627 publications, 38.23%), United Kingdom (395 publications, 24.08%), and Thailand (294 publications, 17.94%), with total citations of 25 280, 18 622 and 15 474, respectively, and the most productive countries included Mahidol University (Thailand), Oxford University (England) and London University (England), with 234, 220 publications and 142 publications and 15 058, 15 421 citations and 6 191 citations, respectively. The three most productive authors were all from Mahidol University, with 85, 77 and 63 publications, respectively; and among the three most cited authors, two were from Mahidol University, Thailand, with 8 623 and 7 961 total citations, and one from National Institutes of Health, the United States of America, with 6 267 total citations. A total of 138 articles were published by Chinese scientists, with 3 434 total citations, and National Institute of Parasitic Diseases of Chinese Center for the Diseases Control and Prevention was the most productive Chinese institution, with 35 publications, 1 165 total citations and 33.29 citations per publication. Conclusions The literature output of ART resistance in P. falciparum was relatively high in the United States of America, Europe, and Southeast Asian countries during the period from 2011 to 2021, with a relatively high academic impact. Publications in Malaria Journal and Antimicrobial Agents and Chemotherapy are recommended to be paid much attention by Chinese scientists to understand the latest advances and extend the research interests.

The ratio of superior mesenteric artery diameter to superior mesenteric vein diameter based on non-enhanced computed tomography in the early diagnosis of spontaneous isolated superior mesenteric artery dissection / 世界急诊医学杂志（英文）

@#BACKGROUND: Spontaneous isolated superior mesenteric artery (SMA) dissection (SISMAD) is a rare cause of abdominal pain. The aim of the study is to investigate the role of a new parameter, the ratio of the SMA diameter to the superior mesenteric vein (SMV) diameter (SMA/SMV) based on non-enhanced computed tomography (CT), in the early diagnosis of SISMAD. METHODS: In a registry study from December 2013 to June 2021, 97 abdominal pain SISMAD patients (SISMAD group) admitted to our hospital were enrolled. Meanwhile, the matched sex and age abdominal pain non-SISMAD patients at 1:2 were collected in reverse chronological order as the control group. Student's t-test, Wilcoxon rank-sum test, and Chi-square test were used to compare differences between the SISMAD and control groups. MedCalc was used to generate receiver operating characteristic (ROC) curve. RESULTS: A total of 291 abdominal pain patients, including 97 SISMAD patients and 194 non-SISMAD patients, were included in the current study. The maximum SMA diameter, perivascular exudation, and SMA/SMV based on non-enhanced CT were significant between the two groups (all P<0.05). ROC curves showed that for the maximum SMA diameter, the area under the curve (AUC), cut-off, sensitivity, and specificity were 0.926, 9.80, 93.8%, and 79.4%, respectively. For SMA/SMV, its AUC, cut-off, sensitivity, and specificity were 0.956, 0.83, 88.7%, and 92.3%, respectively. The diagnostic efficiency of SMA/SMV was better than that of the maximum SMA diameter (P<0.05). The combined parameters of SMA/SMV and maximum SMA diameter had the best diagnostic efficiency (AUC=0.970). CONCLUSION: SMA/SMV may be a potential marker for SISMAD.

Research on the influence of sport dance on physical health in national fitness exercise / Pesquisa sobre a influência da dança esportiva na saúde física no exercício de condicionamento físico nacional / Investigación sobre la influencia de la danza deportiva en la salud física en el ejercicio de acondicionamiento físico nacional

ABSTRACT Introduction: Sports dance is widely known as a competitive game, but as a leisure activity, there is little research on the efficacy of human health and fitness. Sports dance, as a popular national fitness exercise, has sound health promotion effects. At present, domestic and foreign researches mostly focus on the impact of sports dance on young men and women. Objective: This study will explore the influence of regular physical dance exercises on the body shape, function, and quality of middle-aged and older adults, provide a scientific basis for the role of physical dance in national fitness. Methods: This study recruited 20 healthy middle-aged and elderly members of a leisure sports dance club without professional dance experience for three months of dance training. The first two weeks are pre-experiments five times a week. Each exercise time is 60 minutes. The last ten weeks are formal experiments, two times a week, 90 minutes each time, and no other physical exercises are involved in everyday life. By comparing part of the body shape, physical function, and physical fitness index before and after the experiment, the impact of sports dance on middle-aged and older adults' healthy physical fitness is evaluated. Results: After three months of physical dance exercise with different dance styles, in male, BMI index, body fat percentage, waist circumference, hip circumference, and thigh circumference indicators all decreased, but there was no significant difference. In women, BMI index, body fat percentage (P<0.01), waist circumference (P<0.05), diastolic blood pressure (P<0.01), sitting body forward bending (P<0.05), and standing with one foot and closed eyes all increased. In women, the indexes of grip strength (P<0.01), forward bending in sitting position (P<0.055), and standing with eyes closed on one foot all increased. Conclusions: It is suggested that long-term moderate-intensity aerobic sport dance exercise can improve the body shape of middle-aged and older adults and help increase physical fitness; meanwhile, sports dance can effectively improve the cardiovascular function of middle-aged and elderly subjects. Level of evidence II; Therapeutic studies - investigation of treatment results.

RESUMO Introdução: A dança esportiva é amplamente conhecida como um jogo competitivo, mas como atividade de lazer, existem poucas pesquisas sobre sua eficácia na saúde e forma física humana. A dança esportiva, como exercício físico popular em âmbito nacional, tem importantes efeitos na promoção da saúde. Atualmente, a pesquisa nacional e estrangeira está focada principalmente no impacto da dança esportiva em jovens de ambos os sexos. Objetivo: Este estudo irá explorar a influência dos exercícios regulares de dança física na forma, função e qualidade do corpo de adultos mais velhos e de meia-idade, e fornecerá uma base científica para o papel da dança física no fitness nacional. Métodos: Este estudo recrutou 20 pessoas saudáveis, de meia-idade e idosos de um clube esportivo sem experiência profissional em dança durante três meses de treinamento. As primeiras duas semanas foram pré-experimentos cinco vezes por semana. Cada tempo de exercício foi de 60 minutos. As últimas dez semanas foram experimentos formais, duas vezes por semana, 90 minutos cada vez, sem nenhum outro exercício físico na vida cotidiana. Ao comparar a forma corporal, a função física e o índice de aptidão física antes e depois do experimento, o impacto da dança esportiva na aptidão física saudável de adultos mais velhos e de meia-idade é avaliado. Resultados: Após três meses de exercício físico de dança com diferentes estilos de dança, nos homens, o índice de IMC, o percentual de gordura corporal, a circunferência da cintura, a circunferência do quadril e a circunferência da coxa diminuíram, mas não houve diferença significativa. Nas mulheres, o índice de IMC, o percentual de gordura corporal (P <0,01), a circunferência da cintura (P <0,05), a pressão arterial diastólica (P <0,01), o corpo sentado inclinado para frente (P <0,05) e com um pés e olhos fechados aumentaram. Nas mulheres, os índices de força de preensão (P <0,01), de inclinação para a frente na posição sentada (P <0,055) e com um pé com os olhos fechados aumentaram. Conclusões: Sugere-se que o exercício de dança esportiva aeróbica de intensidade moderada de longa duração pode melhorar a forma corporal de adultos mais velhos e de meia idade e ajudar a aumentar a aptidão física; enquanto isso, a dança esportiva pode efetivamente melhorar a função cardiovascular de indivíduos de meia-idade e idosos. Nível de evidência II; Estudos terapêuticos: investigação dos resultados do tratamento.

RESUMEN Introducción: La danza deportiva es ampliamente conocida como un juego competitivo, pero como actividad de ocio hay poca investigación sobre la eficacia en la salud y el estado físico humanos. La danza deportiva, como ejercicio físico popular a nivel nacional, tiene importantes efectos en la promoción de la salud. En la actualidad, las investigaciones nacionales y extranjeras se centran principalmente en el impacto de la danza deportiva en hombres y mujeres jóvenes. Objetivo: Este estudio explorará la influencia de los ejercicios regulares de danza física en la forma, función y calidad del cuerpo de los adultos mayores y de mediana edad, y proporcionará una base científica para el papel de la danza física en la aptitud nacional. Métodos: Este estudio reclutó a 20 miembros sanos de mediana edad y ancianos de un club deportivo sin experiencia profesional en danza durante tres meses de entrenamiento. Las dos primeras semanas eran experimentos previos cinco veces por semana. Cada tiempo de ejercicio fue de 60 minutos. Las últimas diez semanas eran experimentos formales, dos veces por semana, 90 minutos cada vez, sin otros ejercicios físicos en la vida cotidiana. Al comparar la forma del cuerpo, la función física y el índice de aptitud física antes y después del experimento, se evalúa el impacto de la danza deportiva en la aptitud física saludable de los adultos mayores y de mediana edad. Resultados: Después de tres meses de ejercicio físico de baile con diferentes estilos de baile, en los hombres, el índice de IMC, el porcentaje de grasa corporal, la circunferencia de la cintura, la circunferencia de la cadera y la circunferencia del muslo disminuyeron, pero no hubo diferencia significativa. En las mujeres, el índice de IMC, el porcentaje de grasa corporal (P <0.01), la circunferencia de la cintura (P <0.05), la presión arterial diastólica (P <0.01), el cuerpo sentado inclinado hacia adelante (P <0.05) y con un pie y ojos cerrados aumentaron. En las mujeres, aumentaron los índices de fuerza de agarre (P <0.01), de inclinación hacia adelante en posición sentada (P <0.055) y con un pie con los ojos cerrados. Conclusiones: Se sugiere que el ejercicio de danza deportiva aeróbica de intensidad moderada a largo plazo puede mejorar la forma del cuerpo de los adultos mayores y de mediana edad y ayudar a aumentar la aptitud física; mientras tanto, la danza deportiva puede mejorar eficazmente la función cardiovascular de sujetos de mediana edad y ancianos. Nivel de evidencia II;Estudios terapéuticos: investigación de los resultados del tratamiento.

Does public pension impact migrant workers' nutrition: evidence from urban pension insurance in China / A previdência pública impacta a nutrição de trabalhadores migrantes: evidências do seguro de pensão urbana na China

ABSTRACT: Based on the data of "employment and food demand of urban migrant workers", this paper empirically analyzed the impact of urban pension insurance on the nutritional intake structure of migrant workers. The results showed that participating in urban pension insurance can change the nutritional intake structure of migrant workers. Additionally, fat and protein replace carbohydrate as the main nutrition sources for migrant workers. After controlling the income and labor intensity of migrant workers and other factors, urban pension insurance has a positive effect on the intake of fat and protein of migrant workers for they increase by 13.5% and 8.8% respectively. There is no significant effect on the intake of carbohydrates of migrant workers. The calorie intake of migrant workers increases by 6.8% accounting for the change of nutritional intake structure. Endogenous and robustness tests showed that the above conclusions are robust. Heterogeneity analysis showed that there is no significant difference in the effect of urban pension insurance on calorie intake of migrant workers in different income levels and age groups.

RESUMO: Este artigo usa os dados da pesquisa de "Emprego e demanda alimentar dos trabalhadores migrantes urbanos" para analisar empiricamente o impacto do seguro patrimonial urbano na estrutura de ingestão nutricional dos trabalhadores migrantes. O estudo constatou que: o seguro-pensão urbano mudou a estrutura de ingestão nutricional dos trabalhadores migrantes. Gordura e proteína substituíram os carboidratos e se tornaram a principal fonte de nutrição dos trabalhadores migrantes. Depois de controlar fatores como a renda e a intensidade do trabalho dos trabalhadores migrantes, o seguro de pensão urbana aumentou significativamente a ingestão de gordura e proteína dos trabalhadores migrantes em 13,5% e 8,8%, respectivamente, enquanto a ingestão de carboidratos dos trabalhadores migrantes não foi afetada. Significativamente afetado. A transformação da estrutura de ingestão nutricional dos trabalhadores migrantes aumentou sua ingestão calórica em 6,8%. Os testes de endogenia e robustez mostram que as conclusões acima são robustas. A análise de heterogeneidade mostra que não há diferença significativa no efeito do seguro previdenciário urbano sobre o aumento da ingestão de calorias para trabalhadores migrantes de diferentes níveis de renda e diferentes grupos etários.

Arthroscopic Bankart repair combined with Reimplissage for the treatment of recurrent anterior dislocation of shoulder in adults with Hill-Sachs defect / 中国骨伤

OBJECTIVE@#To investigate the method and clinical effects of the treatment of recurrent anterior dislocation of shoulder with Hill-Sachs injury by arthroscopic Bankart repair and Remplissage.@*METHODS@#From March 2016 to March 2019, 106 patients with recurrent anterior dislocation of shoulder with glenoid bone defect less than 20% underwent arthroscopic Bankart repair, including 76 males and 30 females, aged from 18 to 45 (27.3±8.6) years, 59 cases of left shoulder and 47 cases of right shoulder. Range of motion (ROM), American Shoulder and Elbow Surgeons(ASES) score, Constant-Murley score and Rowe score were used to evaluate shoulder functionand stability before and after operation.@*RESULTS@#All patients were followed up, and the duration ranged from 21 to 60 months, with a mean of (41.5± 8.5) months. One patient developed infection after operation, and the infection was controlled after arthroscopic debridement again. The remaining patients did not have clinical complications such as infection, intra articular hematocele and redislocation. Shoulder flexion and lifting increased from (158.33±15.72) ° preoperatively to (169.43±10.04) ° at the latest follow up, and internal rotation changed from T7 (T4 to T10) preoperatively to T8 (T5 to T10) at the latest follow up;the average lateral external rotation and abduction 90 ° external rotation decreased from (58.46±15.51) ° preoperatively and (99.37±14.09) ° to (53.18±14.90) ° and (92.52±13.10) ° at the latest follow up, respectively. The ASES score, Constant -Murley score and Rowe score were significantly improved.@*CONCLUSION@#The clinical effect of rehabilitation of Bankart repair combined with Remplissageunder arthroscopy in the treatment of recurrent dislocation of shoulder joint in adults with Hill-Sachs defect is satisfactory. Although the external rotation function is weaker than that before operation, it can effectively reconstruct the shoulder function and avoid the occurrence ofdislocation after operation.

Study on the metabonomics of vitamin A deficiency urine of left-behind children under 1 year old in Zunyi area based on hydrogen-nuclear magnetic resonance / 中华实用儿科临床杂志

Objective:Explore the hydrogen-nuclear magnetic resonance ( 1H-NMR) application potential of metabolomics in early screening of vitamin A deficiency(VAD). Methods:From January to June 2019, 40 cases of left-behind children with confirmed VAD <1 year old were enrolled in Zunyi area as VAD group, and 40 healthy children were selected as healthy control group.Urine from the 2 groups of children was collected and 1H-NMR spectrum was measured.By combining metabonomics technology and multivariate statistical analysis, the 2 groups of metabolites were compared, and the differential metabolites of children with VAD were screened out. Results:Compared with VAD group [(0.56±0.10)μmol/L], the difference in the serum retinol concentration of healthy control group [(1.35±0.18)μmol/L] was statistically significant ( t=23.93, P<0.001). Compared with healthy group, VAD group showed higher level of methylmalonate, 2-ketoisovalerat, N-acetyl glycoprotein signal, dimethylglycine and betaine, with statistically significant differences (| r|>0.602, all P<0.05). The level of Isovalerylglycine, 2-hydroxyisobutyrate, creatine, phosphate choline/glycerophosphocholine, 4-PY and Hippurate were significantly decreased, with statistically significant differences (| r|>0.602, all P<0.05). Conclusions:Compared with healthy children, children with VAD have differences in urine Metabolites.Differential metabolites are closely correlated with intestinal microecological ba-lance, digestive system diseases, respiratory system diseases, immune-related diseases, energy metabolism, growth and development.Urine 1H-NMR metabolomics analysis has potential application value in the early screening of VAD.

Effect of cell sheet combined with 3D printing an antler powder/silk fibroin/polyvinyl alcohol scaffold on the repair of mandibular defects in sheep / 口腔疾病防治

Objective @#To explore the feasibility of antler powder/silk fibroin/polyvinyl alcohol scaffolds as tissue engineering bone scaffolds and the relationship between their degradation performance and the healing speed of bone defects.@*Methods @# Antler powder/silk fibroin/polyvinyl alcohol scaffolds and nano hydroxyapatite/silk fibroin/polyvinyl alcohol scaffolds were prepared by 3D printing. The whole bone marrow culture method was used to prepare blood cell sheets of Altay big tail sheep’s iliac bone marrow. With observation times of 1, 2 and 3 months, the mandibular defects of 4 sheep were established. The experimental group was coated with antler powder/silk fibroin/polyvinyl alcohol scaffolds. The control group was coated with nanohydroxyapatite/silk fibroin/polyvinyl alcohol scaffolds. The negative control group was coated with gel-free sponges. According to the self-control method of the bilateral mandible defect area, scaffolds wrapped with cell membranes or gel sponges wrapped with cell membranes were implanted. At the ends of the first, second and third months after implantation, the experimental animals were killed, cone beam CT was performed, and paraffin sections were taken for HE staining to evaluate the effect of different scaffold materials on bone regeneration and scaffold degradation.@* Results@# Scanning electron microscopy showed that both groups had regular pores and good continuity, and there was no difference in pore size and porosity between the two groups (P > 0.05). The results of CBCT imaging showed that in 3 months after operation, the experimental group had significantly better repair effects on bone defects than the control group, and the degradation rate matched the bone repair rate. The bone mineral density in the center of the defect was higher than that of the control group, which was close to that of normal bone tissue. The central bone mineral density of the experimental group at each time point was higher than those of the control group and the negative control group, and the difference was statistically significant (P < 0.05). HE staining results showed that the bone cells in the experimental group were more active, with more new capillaries and bone trabeculae formed, and the scaffold material absorbed more than the control group. @*Conclusion @#The antler powder/silk fibroin/polyvinyl alcohol scaffold can promote the repair of critical bone defects. Its degradability matches its bone tissue healing rate. It is expected to become a promising scaffold material for bone tissue engineering.

A newly designed biliary brachytherapy drainage catheter for patients with malignant biliary obstruction: A pilot study

Aim: Self.expandable metallic stent (SEMS) placement has been considered as the preferred treatment to relieve jaundice in nonsurgical patients. However, 50% of stents become stenosed within 3.6 months due to tumor ingrowth and epithelial hyperplasia. This study aims to evaluate the feasibility and efficacy of a newly designed brachytherapy biliary drainage catheter (BBDC) loaded with 125I seeds for palliation of malignant biliary obstruction (MBO). Methods: In this prospective study, patients with unresectable MBO underwent BBDC placement after SEMS placement at our center from September 2017 to April 2019. Results: A total of 21 patients with MBO were enrolled. The technical and clinical success rates were 100%. Total bilirubin, direct bilirubin, alanine aminotransferase, alkaline phosphatase, cancer antigen 19.9, and carcinoembryonic antigen levels significantly decreased during the 1.month follow.up (P < 0.05). Four patients (19%) had minor complications. During the median follow.up of 299 days, 13 patients (61.9%) developed stent occlusion. The 6.month stent patency and survival rates were 73.5% and 79.2%, respectively. The median stent patency and survival were 279 and 454 days, respectively. Conclusion: The use of BBDC loaded with 125I seeds is a feasible and effective method to prolong biliary stent patency in patients with MBO

The correlation between inflammation levels and lung function on patients defined by different TCM syndromes with acute exacerbation of chronic obstructive pulmonary disease / 国际中医中药杂志

Objective:To observe the changes of neutrophil to lymphocyte ratio and lung function of patients defined by different TCM syndromes with acute exacerbation of chronic obstructive pulmonary disease.Methods:A total of 181 patients with acute exacerbation of chronic obstructive pulmonary disease in our hospital from January 2016 to Novenber 2018 were included and divided into 5 groups accoding to different TCM syndromes, which were wind-cold invading lung syndrome (26), external cold and internal fluid syndrome (40), phlegm-heat obstructing lung syndrome (48), phlegm-dampness blocked syndrome (48), an heart spirit confused by phlegm syndrome (19). Then tested the blood for each group, and calculated NLR; tested c-reactive protein, and lung function, and measured with COPD Assessment Test (CAT) and Modified British Medical Research Council (mMRC); conducted 6MWT, then the BODE index and Charlson Comorbidity Index (CCI) scores were calculated.Results:The NLR, CRP level in patients with external cold and internal fluid syndrome and an heart spirit confused by phlegm syndromeby phlegm were significantly higher than those of other syndrome ( P<0.05 or P<0.01), and FEV1% Pred was significantly lower than that of phlegm-dampness blocked syndrome ( P=0.024); the 6-minute walking distance of patients with external cold and internal fluid syndrome was significantly shorter than that of phlegm-heat obstructing lung syndrome and phlegm-dampness blocked syndrome ( P=0.006, P=0.024). The comparison of mMRC grades among the syndromes was statistically significant ( F=26.367, P=0.049). The BODE index of patients external cold and internal fluid syndrome was higher than that of patients phlegm-heat obstructing lung syndrome ( P=0.015), and the CCI score of patients an heart spirit confused by phlegm syndrome was higher than that of patients phlegm-dampness blocked syndrome ( P=0.012). The BODE index of patients with external cold internal fluid syndrome was significantly higher than that of phlegm-heat obstructing lung syndrome ( P=0.015). The CCI score of patients with mental disorder caused by phlegm was significantly higher than that of phlegm-dampness blocked lung syndrome ( P=0.012). Conclusions:There were significantly differences in neutrophil to lymphocyte ratio, CRP and 6-minute walking distance among different TCM syndromes in patients with acute exacerbation of chronic obstructive pulmonary disease. The inflammation in patients with external cold and internal fluid syndrome and mental disorder caused by phlegmare much severe, but there might be no difference in dyspnea and prognosis among TCM syndromes.

Construction of rat diacylglycerol kinase gamma lentivirus overexpression vector by homologous recombination / 中国组织工程研究

BACKGROUND: Lentiviral vectors have been widely used as exogenous transgenic vectors. However, a recombinant lentiviral vector containing rat diacylglycerol kinase y (DGKy) gene has not been reported. OBJECTIVE: To construct lentiviral overexpression vector of rat DGKy by homologous recombination. METHODS: Total RNA was extracted from the brain tissue of adult Sprague-Dawley rats, and the cDNA obtained by PCR was used as a template to amplify the 5'-end 1 029 bp and the 3'-end 1 362 bp of the rat DGKy gene CDS. Then, the two homologous recombination fragments were ligated into the plasmid vector. The positive clones were confirmed by PCR and DNA sequencing. The CMV-rat DGKy-GFP lentiviral vector and the lentiviral packaging system were co-transfected into 293T cells for virus packaging and lentivirus was collected to infect 293T cells. The expression of GFP in infected 293T cells was observed under fluorescence microscope. Real-time PCR and western blot assay were used to detect the relative expression of DGKy mRNA in infected 293T cells. RESULTS AND CONCLUSION: The results of PCR simplification and sequencing indicated that the CMV-rat DGKy-GFP lentiviral vector was successfully constructed. In 293T cells infected with CMV-rat DGKy-GFP lentivirus, the expression of GFP was observed under fluorescence microscope and the DGKy mRNA expression was increased significantly than that of the vector group by real-time PCR (P < 0.01). Western blot assay results showed that the DGKy protein expression of the selected GFP-positive 293T cells was increased very significantly (P < 0.001). To conclude, the rat DGKy lentiviral overexpression vector has been successfully constructed and maintains high expression in 293T cells.

Gambogic acid enhances sensitivity of glioma U251 cells to temozolomide by inhibiting GLUT-3/ AKT signaling pathway / 中国肿瘤生物治疗杂志

@#[Abstract] Objective: To explore whether gambogic acid can enhance the sensitivity of glioma U251 cells to temozolomide and further explore its mechanism. Methods: U251 cells were cultured in vitro and divided into blank control group, gambogic acid treatment group, temozolomide alone treatment group and combined treatment group. The cells survival rates of cells in each group was detected by CCK-8 assay. Flow cytometry was used to detect cell apoptosis and changes in ROS level. Western blotting was used to detect the changes in protein expressions. Results: CCK-8 results showed that the cells survival rates of the four groups after treatment for 24 h were (98.65±3.68)%, (93.58±2.47)%, (66.81±2.39)% and (38.65±4.13)%, respectively. It can be seen that the combined treatment could significantly increase the inhibitory effect of temozolomide on U251 cells. The proportion of apoptotic U251 cells in the combined treatment group was (61.43±2.58)%, which was significantly higher than that of (26.68±1.82)% in the temozolomide-treated group. Combined treatment of gambogic acid and temozolomide could up-regulate ROS level in U251 cells, reduce the expressions of GLUT-3 and p-AKT, and inhibit the GLUT-3/AKT signaling pathway. Conclusion: Gambogic acid combined with temozolomide can enhance the sensitivity of U251 cells to temozolomide by up-regulating ROS level and inhibiting GLUT-3/AKT signaling pathway in U251 cells, and provides a theoretical basis for the application of gambogic acid in the treatment of glioma.

Study on urine metabolomics of left-behind children with vitamin D deficiency under 1 year old in Zunyi area based on nuclear magnetic resonance / 中华实用儿科临床杂志

Objective@#To explore the characteristic changes in urinary metabolites in left-behind children with vitamin D deficiency under 1 year old in Zunyi area by metabolomic nuclear magnetic resonance (NMR) in order to provide new biomarkers for early diagnosis of vitamin D deficiency.@*Methods@#From January to August 2018, blood tests and urine collection were carried out on the left-behind children under 1 year old in Fenggang county, Bozhou district and Zheng′an county under Zunyi city by stratified sampling.Forty children diagnosed as a vitamin D deficiency were selected as a vitamin D deficiency group, and 40 children with normal urine test were selected as a healthy control group.For urine sampling, SIMCA-P+ software was applied to analyze the integral value of hydrogen spectrogram by principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) was used to distinguish the difference in urine metabolites between two groups of the left-behind children.Orthogonal partial least squares discriminant analysis (OPLS-DA) was used to screen different metabolites.@*Results@#The serum level of 25-hydroxy vitamin D[25-(OH)D][(32.0±3.6) nmol/L ] in the healthy control group was higher than that in the vitamin D deficiency group[(15.8±2.3) nmol/L], and the difference was statistically significant (P<0.05). PCA and PLS-DA analysis showed significant differences in urine metabolites between the healthy control group and the vitamin D deficiency group (P<0.05). OPLS-DA indicated R2X=0.365, Q2=0.978, which further verified the difference of metabolites.Compared with the healthy control group, the urine of methyl malonic acid, 3-hydroxy butyrate, N-acetyl glycoprotein signal, glutamic acid, dimethyl glycine, 2-ketone glutaric acid, taurine, fumaric acid salt level increased significantly in the vitamin D deficiency group, and the differences were statistically significant (|r|>0.602, all P<0.05, df=39). However, the levels of ethyl malonic acid, creatine, choline, glycerophosphalocholine and equine were significantly decreased, and the differences were statistically significant (|r|>0.602, all P<0.05, df=39).@*Conclusions@#The left-behind children under 1 year old with vitamin D deficiency in Zunyi region are mainly characterized by disorder in energy metabolism, lipid metabolism, amino acid metabolism and intestinal microbial meta-bolism disorders, and their differential metabolites have potential application value in early diagnosis and pathogenesis of vitamin D deficiency.

Comparison of diagnostic value of MRI plain scan and DWI for autoimmune pancreatitis / 中国基层医药

Objective@#To compare the diagnostic value of MRI plain scan and DWI for autoimmune pancreatitis(AIP).@*Methods@#From March 2016 to April 2017, 82 AIP patients in the Central Hospital of Taiyuan were selected in the research.And 42 healthy volunteers were selected as the control group.All patients received MRI plain scan and DWI examination.The imaging characteristics of AIP were observed and the diagnostic value of the two methods was compared.@*Results@#Among the 82 AIP patients, 42(51.22%) had diffuse pancreatic enlargement, 21(25.61%) in local pancreas, 72(87.80%) in pancreatic duct stricture, 68(82.93%) in common bile duct strictures, 67(81.71%) in false envelopes, and MRI plain scanning with low T1 signal, high T2 signal and delayed enhancement.The lesion was high in DWI, and the ADC was low.The average ADC value [(1.12±0.15)mm2/s] was significantly lower than that of the normal pancreas [(1.87±0.25)mm2/s], and the difference was statistically significant(t=22.295, P<0.05). The positive predictive value of MRI plain scan was 72.36%, the negative predictive value was 81.37%, and the sensitivity was 71.94%.The specificity was 70.52%.The positive predictive value of DWI for diagnosing AIP was 85.48%, the negative predictive value was 89.13%, and the sensitivity was 87.52%.The specificity was 88.49%.At ADC=0.001 13mm2/s, the sensitivity and specificity of DWI for the diagnosis of AIP were 86.9% and 89.2%, respectively, and the AUC was 0.826 (95% CI: 0.686-1.804).@*Conclusion@#MRI and DWI manifestations of AIP are characteristic, and the diagnostic efficiency of DWI for AIP is higher than MRI plain scan.

Factors associated with medication adherence among with depressive disorder / 中国医师杂志

Objective@#To explore the status of medication adherence among patients with depressive disorder and analyze its potential influencing factors.@*Methods@#A total of 406 patients with depression who visited our hospital from January 2015 to December 2017 were selected as subjects of study. General data of patients were collected at the time of consultation, and drug adherence questionnaire was used to score the patients at 6 months of follow-up. According to the score, 406 patients were divided into high adherence group, middle adherence group and low adherence group, the correlation between medication adherence and humanistic factors, chronic diseases and course of disease was analyzed.@*Results@#Compared with the low adherence group, the proportion of age (>60 years old), education (<6 years), no spouse, peasant, monthly income (<3 000 yuan), self-funded medical treatment, insomnia history, solitude and negative life events decreased significantly in the middle and high adherence groups. Cadre ratio and the proportion of workers in the middle adherence group increased significantly (P<0.05). Compared with the low adherence group, the course of disease in the middle and high adherence group was significantly shorter (P<0.01). Logistic regression analysis showed that education < 6 years, no spouse, farmers, low income, self-paid medical treatment, solitude, negative life events, course of disease were independently correlated with low adherence of depression patients (P<0.05 or P<0.01).@*Conclusions@#Depression patients have low adherence with medication, so targeted health education should be carried out. Especially for patients with a long course of illness, continuous nursing and long-term follow-up should be done well.Mobilizing family support system , caring for and respecting patients can effectively reduce the occurrence of negative life events. It can improve the medication adherence of depressive patients and promote their early recovery.